Characterization and phylogenetics of beta-lactamase Temoneira gene in Escherichia coli of the Bali cattle on Lombok island, Indonesia

This study aims to determine the character and phylogenetics of β-lactamase Temoneira (blaTEM) gene in Escherichia coli isolated from reproductive tract Bali cattle with repeat breeder cases. was conducted June 2021 that used 16 female cases take their fluid using a plastic sheet gun. Isolation coli, Eosin Methylene Blue Agar identified by biochemical tests. Antibiotic susceptibility test on E. samples carried out disc diffusion method. The screening for presence extended-spectrum done double-disk approximation Characterization blaTEM E PCR method sequences gene, were phylogenetically analyzed. results showed 4 isolates obtained fluid. sensitivity antibiotics 50% resistant penicillin cefotaxime. Samples cefotaxime synergy test. encoded which located at 560 bp position gel electrophoresis. phylogenetic tree analysis found related encoding blaTEM-206 gene. β-lactamase-producing coli. S2.2-EK pEC-S2.2 class A broad-spectrum B-lactamase TEM-206 high degree kinship.